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Growth and biochemical composition of Chlorella vulgaris in different growth media Anais da ABC (AABC)
CHIA,MATHIAS A.; LOMBARDI,ANA T.; MELãO,MARIA DA GRAÇA G..
The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Lipid class; Microalgae; Proteins.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401427
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Taxonomic value of histochemical features of the style in early lineages of Asteraceae Acta Botanica
Hernández,Marcelo Paulo; Katinas,Liliana; Arambarri,Ana María.
In this paper we examine lipophilic substances, tannins, proteins, reducing carbohydrates, starch, and oxalate salts in early lineages of Asteraceae to test if they are a useful complement to classification studies. Styles of mature flowers of 44 species were processed according to plant histochemical techniques and observed by means of a light microscope. Lipophilic compounds were always present in the cuticle and cell walls, and in some species were located inside the cells. Proteins were located in the style cell walls and cell interior of most of the analyzed species. No tannins were found. In general, there is a correlation in the presence of reducing sugars and proteins. Starch granules and oxalate salts were found in the style parenchyma of a few...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Compositae; Crystal salts; Lipophilic substances; Proteins; Starch; Sugars; Taxonomy.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400575
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Evaluation of protein extraction methods for enhanced proteomic analysis of tomato leaves and roots Anais da ABC (AABC)
VILHENA,MILCA B.; FRANCO,MÔNICA R.; SCHMIDT,DAIANA; CARVALHO,GISELLE; AZEVEDO,RICARDO A..
Proteomics is an outstanding area in science whose increasing application has advanced to distinct purposes. A crucial aspect to achieve a good proteome resolution is the establishment of a methodology that results in the best quality and wide range representation of total proteins. Another important aspect is that in many studies, limited amounts of tissue and total protein in the tissue to be studied are found, making difficult the analysis. In order to test different parameters, combinations using minimum amount of tissue with 4 protocols for protein extraction from tomato (Solanum lycopersicum L.) leaves and roots were evaluated with special attention to their capacity for removing interferents and achieving suitable resolution in bidimensional gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bidimensional electrophoresis; Proteins; Protocol optimization; Tomato.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401853
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A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats BJMBR
Fernandes,A.A.; Faria,T. de O.; Ribeiro Júnior,R.F.; Costa,G.P.; Marchezini,B.; Silveira,E.A.; Angeli,J.K.; Stefanon,I.; Vassallo,D.V.; Lizardo,J.H..
Resistance training evokes myocardial adaptation; however, the effects of a single resistance exercise session on cardiac performance are poorly understood or investigated. This study aimed to investigate the effects of a single resistance exercise session on the myocardial contractility of spontaneously hypertensive rats (SHRs). Male 3-month-old SHRs were divided into two groups: control (Ct) and exercise (Ex). Control animals were submitted to sham exercise. Blood pressure was measured in conscious rats before the exercise session to confirm the presence of arterial hypertension. Ten minutes after the exercise session, the animals were anesthetized and killed, and the hearts were removed. Cardiac contractility was evaluated in the whole heart by the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiac; Contractility; Myocardium; Proteins; Resistance exercise.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900813
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Drying kinetics of fermented grape pomace: Determination of moisture effective diffusivity AGRIAMBI
Deamici,Kricelle M.; Oliveira,Lucas C. de; Rosa,Gabriela S. da; Oliveira,Elizangela G. de.
ABSTRACT The aim of this study was to obtain the equilibrium moisture content of grape (variety ‘Tannat’) pomace through desorption isotherms, to evaluate the drying kinetics, determine the coefficient of effective diffusivity and physico-chemically characterize the grape pomace and the product obtained after drying. The desorption isotherms were determined at 50, 60 and 70 ºC and the experimental data were fitted using the GAB model (Gugghenheim, Anderson and de Boer). Drying was evaluated using a 22 factorial experimental design with three center points and effective diffusivity was obtained through the diffusion model of Fick’s second law. The grape pomace was characterized regarding the contents of moisture, protein, carbohydrates, lipids, ash and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Byproducts; Isotherms; Proteins; Fibers.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800763
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The morphological characterization of the dry seeds and reserve mobilization during germination in Morinda citrifolia L. Rev. Ciênc. Agron.
Paula,Stelamaris de Oliveira; Sousa,João Alencar de; Brito,Edy Sousa de; Gallão,Maria Izabel.
ABSTRACT Information about the morphology, chemical composition and reserve mobilization is important in understanding the establishment of native and exotic species. The purpose of this study was to describe the morphology, chemical composition, and mobilization of reserves during germination of noni (Morinda citrifolia L.). Biometric and morphological analyzes were performed with 100 randomly selected dried seeds. Other seeds were treated with sulfuric acid PA and soaked in Petri dishes. Collected seeds of five different times of germination were used for cytochemical and chemical analysis. For cytochemical analysis, the cuts of 5μm were submitted to dyes TB at pH 4.0; XP at pH 2.5, reaction of PAS and Sudan IV. The seeds were crushed for chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochemical; Lipids; Fatty acids; Proteins; Germination.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000300556
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Root Proteomic Analysis of Grapevine Rootstocks Inoculated with Rhizophagus irregularis and Fusarium oxysporum f. sp. herbemontis Rev. Bras. Ciênc. Solo
Vilvert,Elisa; Costa,Murilo Dalla; Cangahuala-Inocente,Gabriela Claudia; Lovato,Paulo Emílio.
ABSTRACT Grapevine decline and death caused by the pathogenic fungus Fusarium oxysporum f. sp. herbemontis is among the main phytosanitary problem for viticulture in southern Brazil. The eradication of infected plants is presently the most common procedure for disease control in vineyards. Inoculation with arbuscular mycorrhizal fungi is an option to reduce or neutralize the negative impacts of soil pathogenic microorganisms, but the mechanisms of plant response involved in this process are not yet completely elucidated. In order to better understand these mechanisms, an experiment was carried out to identify proteins related to plant defence induced by the mycorrhizal fungus after infection with the pathogenic fungus. We used the grapevine rootstocks SO4...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis; Mycorrhiza; Pathogenic fungus; Defence response; Proteins.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100401
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Nutritional quality of the protein of Vigna unguiculata L. Walp and its protein isolate Rev. Ciênc. Agron.
Frota,Karoline de Macêdo Gonçalves; Lopes,Lays Arnaud Rosal; Silva,Izabel Cristina Veras; Arêas,José Alfredo Gomes.
ABSTRACT A protein's true digestibility and amino acid composition are important characteristics for its nutritional characterisation. This study determined the true digestibility of the protein from cowpea (Vigna unguiculata L. Walp.) and of its protein isolate, and their nutritional values were estimated after correcting for the amino acid score. The protein was isolated from the defatted whole bean by its alkaline solubilisation and isoelectric precipitation. The amino acid composition of the protein from the whole bean and from the isolate was determined by ion-exchange chromatography. Protein digestibility was assessed by the nitrogen balance method. The amino acid score is the lowest value obtained from the ratio between the individual amount of each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legume; Amino acid composition; Proteins; Digestibility.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000500792
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Beverages formulated with whey protein and added lutein Ciência Rural
Rocha,Juliana de Cássia Gomes; Mendonça,Adriana Corrêa; Viana,Kéllen Wanessa Coutinho; Maia,Mariza de Paiva; Carvalho,Antônio Fernandes de; Minim,Valéria Paula Rodrigues; Stringheta,Paulo César.
ABSTRACT: This study aimed to develop and characterize beverages formulated with whey protein and added lutein. Beverages formulated with 0.5 (F1), 2.0 (F2), 4.0 (F3) and 6.0% w/v (F4) whey protein were physicochemically and microbiologically characterized, and sensory evaluated. The physicochemical analyses indicated that the protein content significantly changed (P<0.05) the acidity values, soluble solids and the colorimetric coordinates, making possible to adjust mathematical models for all these variables. Microbiological analyses showed no significant contamination (P<0.05) during processing that would compromise drinks quality of the drinks. Carotenoid content and the antioxidant activity did not change significantly (p>0.05) with increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant activity; Lutein; Proteins; Protein drink; Sensory acceptance; Whey.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300752
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Ecological data for western Indian Ocean tuna ArchiMer
Bodin, Nathalie; Chassot, Emmanuel; Sardenne, Fany; Zudaire, Iker; Grande, Maitane; Dhurmeea, Zahirah; Murua, Hilario; Barde, Julien.
Tipo: Text Palavras-chave: Energetics; Fatty acids; Lipids; Morphometrics; Multi-tissues; Proteins; Stable isotopes; Trophic ecology; Tropical marine ecosystems; Tuna fisheries.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00626/73787/75067.pdf
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Growth performance, morphometric analysis of the intestinal mucosa and thyroid of broiler fed canola meal Arq. Bras. Med. Vet. Zootec.
Moraes,P.O.; Novelini,L.; Krabbe,E.L.; Kawski,V.L.; Nunes,J.K.; Corcini,C.D.; Varella,A.S.; Xavier,E.G..
ABSTRACT The aim of this study was to evaluate the effect of replacing soybean meal with canola meal in broiler diets on performance, liver histopathology, morphometry of the intestinal mucosa and thyroid. One-day-old Cobb chicks (n=300) were distributed in a completely randomized design, with increasing levels of replacement of soybean meal with canola meal (0, 25, 50, 75 and 100%) and 6 repetitions of 10 birds each. Weight gain decreased linearly (P< 0.05) with increasing levels of inclusion of canola meal, in all stages. Villus height in the duodenum and jejunum linearly decreased (P< 0.05). Follicle diameter and thyroid follicular epithelium height increased linearly with increasing levels of canola meal (P< 0.05). Similarly, there was an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrition; Poultry; Proteins; Thyroid gland.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100187
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Nova Estratégia Metodológica para Análise Eletroforética (Tricina-SDS-PAGE), visando à Identificação Peptídeo-proteica em Soja. Infoteca-e
STEPHAN, M. P.; FELBERG, I.; SANTOS, A. A. dos; AZEVEDO, T. de L.; CARRÃO-PANIZZI, M. C..
Recentemente, tem sido mostrado o potencial medicinal de peptídeos bioativos oriundos tanto da soja amarela quanto da preta. Publicações sobre a caracterização molecular de hidrolisados proteicos de soja amarela por eletroforese em gel de poliacrilamida (SDS-PAGE) não mostram a visualização de peptídeos na faixa de 10 kDa e 1 kDa. Nesse trabalho, desenvolveu-se uma nova estratégia metodológica de preparo da amostra, junto com a utilização das eletroforeses dos tipos SDS-PAGE e TRIS/TRICINA. Os extratos parcialmente purificados mostraram a obtenção de um gel límpido contendo cadeias peptídicas-proteicas bem separadas. Como destaque foi evidenciada uma proteína com massa acima de 100 kDa e um peptídeo de 11 kDa, somente para a soja preta. Esta técnica de...
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Caracterização molecular; Extrato de soja; Eletroforese; Proteína; Peptídeo; Tecnologia de Alimento; Food technology; Proteins; Peptides; Soy protein.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1117697
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Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) Rev. Bras. entomol.
Mendonça,Angélica; Bernardi Marchiotti,Rafaella C.; Firmino,Ellen L.B.; Santos,Pollyanna P.; Sguarizi Antonio,Denise; Serrão,José E.; Cardoso,Claudia A.L.; Antonialli Junior,William F..
ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mass spectrometry; MALDI-TOF/TOF; Proteins; Wasps.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000400322
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Physiological and biochemical changes during desiccation tolerance loss in millet (Pennisetum glaucum L.) seeds Agronomy
Fernandes, Joana Souza; Guimarães, Renato Mendes; Faria, Jose Marcio Rocha; Pereira, Diego de Sousa; Coelho, Stefania Vilas Boas; Oliveira, Ariadne Santos.
The aim of this study was to evaluate the physiological and biochemical changes related to desiccation tolerance loss in millet seeds. The studied points of the germination process were determined according to the seed imbibition curve of the millet hybrid ADRF6010: control (0h), 3h of imbibition, 1 and 3 mm radicle. The seeds were dried on silica gel for 72h at 20°C, followed by pre-humidification at 25°C for 24h. Seed physiological quality was evaluated by electrical conductivity and a germination test, and seed vigor was evaluated with a first germination count and a germination speed index. The experiment was performed in a completely randomized design, and means were compared by the Scott-Knott test at a 5% probability. The enzymatic systems of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation sensitivity; Germination; Proteins; Enzymes.; Agronomy; Dessecação de sementes; Fisiologia de Sementes; Tolerância à dessecação em sementes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36522
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Physiological and biochemical changes during the loss of desiccation tolerance in millet (Pennisetum glaucum L.) seeds Agronomy
Fernandes, Joana Souza; Guimarães, Renato Mendes; Faria, Jose Marcio Rocha; Pereira, Diego de Sousa; Coelho, Stefania Vilas Boas; Oliveira, Ariadne Santos.
The aim of this study was to evaluate the physiological and biochemical changes related to the loss of desiccation tolerance in millet seeds. The points studied were determined according to the seed imbibition curve of the hybrid ADRF6010: control (0 h), 3 h, 1 and 3 mm of radicle. The seeds were dried on silica gel for 72 h at 20 °C, followed by pre-humidification at 25 °C for 24 h. The physiological quality was evaluated by the electrical conductivity and germination test, and the seed vigor through the first count and germination speed index. The experiment was performed in a completely randomized design and means were compared by the Scott-knott test at 5% probability. The enzymatic systems evaluated were: superoxide dismutase, catalase, peroxidase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed Physiology; Desiccation Tolerance desiccation sensitivity; Germination; Proteins; Enzymes. Agronomy Dessecação de sementes; Fisiologia de Sementes Tolerância à dessecação em sementes.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/36522
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Proteins and Peptides at the Interfaces of Nanostructures Anais da ABC (AABC)
BRITO,ADRIANNE M.M.; BELLETI,ELISANGELA; MENEZES,LUCIVALDO R.; LANFREDI,ALEXANDRE J.C.; NANTES-CARDOS,ISELI L..
Abstract: The present review focuses on the proteins and peptides at the interfaces of nanostructured metals and semiconductors as a result of their use in synthesis in-situ and functionalization of nanostructures. We start the review with an introduction about the peculiar properties of nanostructured materials and their applications. In the following, the chemical and structural properties of peptides and proteins that allow their use as reducing, stabilizing, and functionalization agents are discussed. Proteins and peptides have not only the chemical groups for the metal ion reducing but also provide templates for directing the crystalline growing of nanostructures to the desired shapes and sizes. Proteins and peptides are also used mainly for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Controlled synthesis; Noble-metals; Nanomaterials; Catalysis; Proteins; Peptides.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700402
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Pentoses Used in Cultures of Synechococcus nidulans and Spirulina paracas: Evaluation of Effects in Growth and in Content of Proteins and Carbohydrates BABT
Freitas,Bárbara Catarina Bastos de; Cassuriaga,Ana Paula Aguiar; Morais,Michele Greque de; Atala,Daniel Ibraim Pires; Costa,Jorge Alberto Vieira.
Abstract The biological assimilation of the sugars present in lignocellulosic residues has gained prominence since these residues are the most abundant and economic residues in nature. Thus, the objective of this work was to determine whether the use of D-xylose and L-arabinose as sources of carbon in Synechococcus nidulans and Spirulina paracas cultures affects the growth and production of proteins and carbohydrates. Kinetic growth parameters, pentose consumption, protein content and carbohydrates were evaluated. Synechococcus nidulans and Spirulina paracas consumed all concentrations of pentose used. The highest cellular concentration (1.37 g.L-1) and the highest protein productivity (54 mg.L-1.d-1) were obtained for Spirulina paracas, which was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabinose; Proteins; Spirulina; Synechococcus; Xylose.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100513
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Changes in hormonal balance as key to reserve degradation after dormancy overcoming in Annona macroprophyllata and Annona purpurea seeds Rev. Bras. Frutic.
Ferreira,Gisela; De-la-Cruz-Chacón,Ivan; González-Esquinca,Alma Rosa.
Abstract Studies on the effect of the applying of phytoregulators to overcome seed dormancy have been carried out in Annonaceae species, thus the endogenous relationship between abscisic acid and gibberellins after the application of phytoregulators needs to be known in these species. Considering the importance of hormonal balance in dormancy mechanisms, the aim of this research was to elucidate how endogenous ABA and GA concentrations change after the application of phytoregulators in seeds and how these changes affect reserve degradation during germination of Annona macroprophyllata and A. purpurea seeds. Seeds were submitted to three conditions: - no soaking, soaking-in-water and soaking-in-GA4+7+benzyladenine. ABA, GA, lipids, proteins, and total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abscisic acid; Gibberellins; Germination; Lipids; Proteins; Soluble sugars; Plant growth regulators; Phytoregulators.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000602002
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Electrophoretic behavior of ewe milk proteins from local breeds Rembi and Ouled-Djellal of the Algerian central steppe Acta Agron. (Palmira)
Zobiri-Illoul,Assia; Yabrir,Benalia; Laoun,Abbas; Mati,Abderrahmane.
Abstract In order to characterize the production of sheep milk in Algeria (North of Africa) and to detect molecular markers related to the constitution of the protein phase of this milk, we proposed to analyze the electrophoretic behaviors of caseins and serum proteins under various migration conditions (native, urea and SDS-PAGE), from milk collected during the first three months of the year, from two breeds Ouled-Djellal and Rembi, living in the central area steppe. The profiles obtained show a great similarity and homogeneity between the different samples of the milk of the two breeds of ewes studied as to the number and intensity of the revealed migration bands. Some of the latter are nevertheless distinguished from cow’s milk by different levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algerian steppe; Electrophoresis; Ewe’s breed; Ewe’s milk; Proteins.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122020000100014
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Isolado proteico obtido de subproduto do processamento da castanha de caju. Infoteca-e
LIMA, J. R.; PINTO, C. O.; MORAES, I. V. M. de; RODRIGUES, M. DO C. P.; LIMA, L. V..
A amêndoa da castanha de caju é o produto com maior valor econômico do cajueiro, rica em proteínas e ácidos graxos poli-insaturados. Entre os subprodutos do beneficiamento da castanha estão as amêndoas quebradas, de onde se extrai o óleo comestível, resultando em uma farinha desengordurada. Neste trabalho descrevem-se as características do isolado proteico da amêndoa da castanha de caju, obtido a partir da farinha desengordurada, por solubilização das proteínas em condições alcalinas e posterior precipitação ácida. O isolado obtido apresentou 86,2% de proteínas e rendimento em massa de 19,0%, tendo carboidratos, lipídeos e cinzas como componentes minoritários. Quanto às características tecnológicas, o isolado apresentou capacidade de absorção de água de...
Tipo: Folhetos Palavras-chave: Anacardium occidentale L; Extração aquosa; Kernel; Tecnologia de Alimento; Amêndoa; Proteína; Proteins; Food technology.
Ano: 2022 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1139043
Registros recuperados: 120
Primeira ... 123456 ... Última
 

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